ETO / RUNX1T1 (AML1T1, CBFA2T1, MTG8, ZMYND2) belongs to a family of evolutionarily conserved nuclear factors that act as a transcriptional corepressor. ETO / RUNX1T1 does not interact directly with DNA but is recruited by transcription factors such as PLZF, BCL6, TAL1/SCL, Gfi1and Heb recruit nuclear co-repressors such as NCoR, SIN3, and SMRT / NCoR2, which in turn interact with histone deacetylase (HDAC) to mediate transcriptional repression. ETO / RUNX1T1 contains four evolutionarily conserved functional domains called nervy homology regions (NHRs) involved in protein-protein interaction. ETO / RUNX1T1 is frequently found as a fusion partner of AML1 in acute myeloid leukemia with the t(8,21) translocation which gives rise to the AML-ETO fusion protein. ETO / RUNX1T1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.
Purification
Affinity Purified
Immunogen
This ETO / RUNX1T1 antibody was raised against a peptide within the internal region of human ETO / RUNX1T1.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.