Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)
Characteristics
The CTBP2 (C-terminal binding protein 2) gene encodes a protein that is a corepressor targeting diverse transcription regulators. It functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses. Isoform 2 is also phosphorylated upon DNA damage, probably by ATM or ATR at Thr179, Ser181 and Ser185. Phosphorylation by HIPK2 on Ser428 induces proteasomal degradation. CTBP2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples.
Purification
Affinity Purified
Immunogen
This CTBP2 antibody was raised against a peptide in the C-terminal region of human CTBP2. This antibody recognizes both isoform 1 and 2.
CTBP2
Reactivity: Human
WB, IF, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.