Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Characteristics
CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the a-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes. CENP-B antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Human samples.
Purification
Affinity Purified
Immunogen
This CENP-B antibody was raised against a recombinant protein corresponding to the C-terminal region of human CENP-B.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.