Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)
Characteristics
BRD8 (Bromodomain-containing protein 8, SMAP2, TRCP120) is a member of the Bromodomain-containing family of chromatin associated proteins. BRD8 is a member of the NuA4 histone acetyltransferase complex, involved in the transcriptional activation of specific genes through the acetylation of histones H2A and H4. BRD8 contains two Bromodomains, a motif that has been demonstrated to facilitate interaction with acetylated histones. BRD8 / SMAP2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human samples.
Purification
Affinity Purified
Immunogen
This BRD8 / SMAP2 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human BRD8.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.