5-Methylcytosine antibody

Catalog No. ABIN6971343

Product Details

Target: 5-Methylcytosine

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Methylated DNA Immunoprecipitation (MeDIP), Immunofluorescence (IF), Dot Blot (DB)

Characteristics: 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis. 5-Methylcytosine (5-mC) antibody (mAb) (Clone A1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunofluorescence and Methyl DNA Immunoprecipitation, it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.

Clone: A1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details

Target: 5-Methylcytosine

Alternative Name: 5-Methylcytosine (5-mC)

Target Type: Chemical