IgE antibody (Biotin)

Catalog No. ABIN6963959

Product Details anti-IgE Antibody (Biotin)

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is conjugated to Biotin

Application: ELISA, ELISpot

Specificity: Native human IgE

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Human IgE

Clone: 107-182-101

Isotype: IgG1, IgG2a, IgG2b

Application Details

Application Notes: For detection of antigen-specific IgE by e.g. indirect ELISA. For enumeration of IgE producing cells using ELISpot in combination with coating mAb 107 .

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IgE antibody (Biotin) (ABIN6963959)

Nyborg, Zacco, Ettinger, Jack Borrok, Zhu, Martin, Woods, Kiefer, Bowen, Suzanne Cohen, Herbst, Wu, Coats: "Development of an antibody that neutralizes soluble IgE and eliminates IgE expressing B cells." in: Cellular & molecular immunology, Vol. 13, Issue 3, pp. 391-400, (2017) (PubMed).

Bundhoo, Paveglio, Rafti, Dhongade, Blumberg, Matson: "Evidence that FcRn mediates the transplacental passage of maternal IgE in the form of IgG anti-IgE/IgE immune complexes." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 45, Issue 6, pp. 1085-98, (2016) (PubMed).

Andersen, Mondal, Preissler, Freed, Stockinger, Bell, Druschel, Louis, Lawrence: "Detection of immunoglobulin isotypes from dried blood spots." in: Journal of immunological methods, Vol. 404, pp. 24-32, (2014) (PubMed).

Wakamatsu, Satake, Igarashi, Dogru, Ibrahim, Okada, Fukagawa, Shimazaki, Fujishima: "IgE and eosinophil cationic protein (ECP) as markers of severity in the diagnosis of atopic keratoconjunctivitis." in: The British journal of ophthalmology, Vol. 96, Issue 4, pp. 581-6, (2012) (PubMed).

Scanlon, Hawksworth, Lane, Mahon: "IL-17A induces CCL28, supporting the chemotaxis of IgE-secreting B cells." in: International archives of allergy and immunology, Vol. 156, Issue 1, pp. 51-61, (2011) (PubMed).

Target Details for IgE

Target: IgE

Abstract: IgE Products

Synonyms: IGH2 antibody, IgE antibody, Gm900 antibody, immunoglobulin heavy chain (epsilon polypeptide) antibody, immunoglobulin heavy constant epsilon antibody, Immunoglobulin heavy constant epsilon antibody, Ighe antibody, IGHE antibody

Gene ID: 3497