TXN antibody (Biotin)

Catalog No. ABIN6963928

Product Details anti-TXN Antibody (Biotin)

Target: TXN (Thioredoxin (TXN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TXN antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Native and recombinant Thioredoxin-1

Cross-Reactivity (Details): The antibody cross-reacts with Trx1 from cow. For quantification of human Trx1 in ELISA, samples should not contain bovine serum.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human Thioredoxin-1

Clone: MT13X3

Isotype: IgG1

Application Details

Application Notes: For quantification of Trx1 in solution e.g. cell culture supernatants and serum/ plasma using ELISA. MT13X3 is recommended as detection mAb in combination with coating mAb MT17R6 . MT13X3 is also suitable for Western blot.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-TXN antibody (Biotin) (ABIN6963928)

Faghih, Rostami-Nejad, Amani, Sadeghi, Pourhoseingholi, Masotti, Zali: "Analysis of IL17A and IL21 Expression in the Small Intestine of Celiac Disease Patients and Correlation with Circulating Thioredoxin Level." in: Genetic testing and molecular biomarkers, Vol. 22, Issue 9, pp. 518-525, (2018) (PubMed).

Mahmoudian, Khalilnezhad, Gharagozli, Amani et al.: "Thioredoxin-1, redox factor-1 and thioredoxin-interacting protein, mRNAs are differentially expressed in Multiple Sclerosis patients exposed and non-exposed to interferon and immunosuppressive ..." in: Gene, Vol. 634, pp. 29-36, (2017) (PubMed).

Reiser, Mathys, Curbo, Pannecouque, Noppen, Liekens, Engman, Lundberg, Balzarini, Karlsson: "The Cellular Thioredoxin-1/Thioredoxin Reductase-1 Driven Oxidoreduction Represents a Chemotherapeutic Target for HIV-1 Entry Inhibition." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147773, (2016) (PubMed).

Lundberg, Curbo, Reiser, Masterman, Braesch-Andersen, Areström, Ahlborg: "Methodological aspects of ELISA analysis of thioredoxin 1 in human plasma and cerebrospinal fluid." in: PLoS ONE, Vol. 9, Issue 7, pp. e103554, (2015) (PubMed).

Target Details for TXN

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin1 (TXN Products)

Gene ID: 7295

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis