IL12 antibody

Catalog No. ABIN6963865

Product Details anti-IL12 Antibody

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL12 antibody is un-conjugated

Application: ELISA, ELISpot

Specificity: Native and recombinant p40 subunit of mouse IL-12 and IL-23. The antibody recognizes p40 monomer, p40 homodimer, IL-12 p70 heterodimer (p40+p35) and IL-23 heterodimer (p40+p19).

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant mouse IL-12 p70

Clone: C17-8

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL12 antibody (ABIN6963865)

Ashrafi, Shapouri, Shirkhani, Mahdavi: "Anti-tumor effects of propranolol: Adjuvant activity on a transplanted murine breast cancer model." in: Biomedicine & pharmacotherapy, Vol. 104, pp. 45-51, (2018) (PubMed).

Yazdi, Varastehmoradi, Faghfuri, Mavandadnejad, Mahdavi, Shahverdi: "Adjuvant Effect of Biogenic Selenium Nanoparticles Improves the Immune Responses and Survival of Mice Receiving 4T1 Cell Antigens as Vaccine in Breast Cancer Murine Model." in: Journal of nanoscience and nanotechnology, Vol. 15, Issue 12, pp. 10165-72, (2017) (PubMed).

Almeida, Breyner, Mahi, Ahmed, Benbouziane, Boas, Miyoshi, Azevedo, Langella, Bermúdez-Humarán, Chatel: "Expression of fibronectin binding protein A (FnBPA) from Staphylococcus aureus at the cell surface of Lactococcus lactis improves its immunomodulatory properties when used as protein delivery vector." in: Vaccine, Vol. 34, Issue 10, pp. 1312-8, (2016) (PubMed).

Tadepalli, Singh, Balakrishna, Murali, Batra: "Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model." in: Molecular immunology, Vol. 71, pp. 34-41, (2016) (PubMed).

Zhang, Wang, Liu, Chen, Li, Yang, An, Ma, Pan, Ma: "Comparison of PLA microparticles and alum as adjuvants for H5N1 influenza split vaccine: adjuvanticity evaluation and preliminary action mode analysis." in: Pharmaceutical research, Vol. 31, Issue 4, pp. 1015-31, (2014) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12 (p70) (IL12 Products)

Gene ID: 16159, 16160

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome