IL-12/IL-23 p40 antibody (Biotin)

Catalog No. ABIN6963861

Product Details anti-IL-12/IL-23 p40 Antibody (Biotin)

Target: IL-12/IL-23 p40

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-12/IL-23 p40 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Specificity: Native and recombinant p40 subunit of IL-12 and IL-23. The antibody recognizes p40 monomer, p40 homodimer, p70 heterodimer (p40+p35) and IL-23 heterodimer (p40+p19).

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-12/-23 (p40) from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of the p40 subunit of human IL-12 and IL-23 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-23

Clone: MT618

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963861 and ABIN7448304 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-12/IL-23 p40 antibody (Biotin) (ABIN6963861)

Masuta, Yamamoto, Natsume-Kitatani, Kanuma, Moriishi, Kobiyama, Mizuguchi, Yasutomi, Ishii: "An Antigen-Free, Plasmacytoid Dendritic Cell-Targeting Immunotherapy To Bolster Memory CD8+ T Cells in Nonhuman Primates." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 6, pp. 2067-2075, (2019) (PubMed).

Weinberger, Joos, Reed, Coler, Grubeck-Loebenstein: "The stimulatory effect of the TLR4-mediated adjuvant glucopyranosyl lipid A is well preserved in old age." in: Biogerontology, Vol. 17, Issue 1, pp. 177-87, (2016) (PubMed).

Min, Wang, Yuan, Kuang, Zhao: "Effects of repeated tuberculin skin testings on immune responses in experimental monkeys." in: Journal of animal science and technology, Vol. 56, pp. 32, (2015) (PubMed).

Wang, Wu, Zhou, Liu, Zhou, Zhu, Song, Zhu, Wang, Xia: "IL12p40 regulates functional development of human CD4+ T cells: enlightenment by the elevated expressions of IL12p40 in patients with inflammatory bowel diseases." in: Medicine, Vol. 94, Issue 10, pp. e613, (2015) (PubMed).

Gille-Johnson, Smedman, Gudmundsdotter, Somell, Nihlmark, Paulie, Andersson, Gårdlund: "Circulating monocytes are not the major source of plasma cytokines in patients with sepsis." in: Shock (Augusta, Ga.), Vol. 38, Issue 6, pp. 577-83, (2013) (PubMed).

Target Details for IL-12/IL-23 p40

Target: IL-12/IL-23 p40

Alternative Name: IL12/23 (p40) (IL-12/IL-23 p40 Products)

Gene ID: 3592, 3593