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CD45RO antibody

CD45RO Reactivity: Human FACS, IF, WB, IHC (p), IP, IHC (fro) Host: Mouse Monoclonal UCHL1 unconjugated

Catalog No. ABIN6961743

Datasheet as PDF

Target See all CD45RO products

CD45RO
Reactivity
66

12

12

11

11
Human
Host
54

12
Mouse
Compatible Secondaries
Clonality
54

12
Monoclonal
Conjugate
10

4

4

3

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD45RO antibody is un-conjugated
Application
51

26

21

13

11

9

8

8

3

3

2

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Chimpanzee

Purification

This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Clone

UCHL1

Isotype

IgG2a kappa
anti-CD45RO (CD45RO) antibody
CD45RO Reactivity: Human, Mouse, Rat WB, IHC (p), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD45RO (CD45RO) antibody
GMP Grade CD45RO Reactivity: Human FACS, IHC, IP Host: Mouse Monoclonal UCHL1 unconjugated

anti-CD45RO (CD45RO) antibody (FITC)
GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 FITC

anti-CD45RO (CD45RO) antibody (PE)
GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 PE

anti-CD45RO (CD45RO) antibody (APC)
GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 APC

anti-CD45RO (CD45RO) antibody (mFluor™450)
GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 mFluor™450

anti-CD45RO (CD45RO) antibody (PE-CF®594)
GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 PE-CF®594

anti-CD45RO (CD45RO) antibody
CD45RO Reactivity: Human, Chimpanzee, Marmoset FACS, IF, IHC (p) Host: Mouse Monoclonal UCHL-1 unconjugated

anti-CD45RO (CD45RO) antibody (FITC)
CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 FITC

anti-CD45RO (CD45RO) antibody (PE)
CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 PE

Application Notes

This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment

0.5 mg/mL

Restrictions

For Research Use only
Buffer

10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

2-8°C

Expiry Date

12 months
Target

CD45RO

Alternative Name

CD45RO (CD45RO Products)

Background

The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and others.The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. The antibody is reported to be cross-reactive for Chimpanzee CD45RO.

Gene ID

5788

UniProt

P08575