IL-12/IL-23 p40 antibody
Reactivity: Mouse
FACS, ELISA, IF, IP, Func, WB
Host: Rat
Monoclonal
C17-8
unconjugated
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- Target See all IL-12/IL-23 p40 products
- IL-12/IL-23 p40
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Reactivity
- Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
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Conjugate
- This IL-12/IL-23 p40 antibody is un-conjugated
- Application
- Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func), Western Blotting (WB)
- Purification
- This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.
- Endotoxin Level
- Low endotoxin: < 0.01 EU/ug
- Clone
- C17-8
- Isotype
- IgG2a kappa
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anti-IL-12/IL-23 p40 antibody (APC)
Reactivity: Mouse FACS Host: Rat Monoclonal C17-8 APC
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- Application Notes
- This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.
- Comment
-
2 mg/mL
- Restrictions
- For Research Use only
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- 2-8°C
- Expiry Date
- 12 months
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- Target
- IL-12/IL-23 p40
- Abstract
- IL-12/IL-23 p40 Products
- Background
- The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein, known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, mac- rophages, and dendritic cells, and has been shown to target naive, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19 - its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the re- ceptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine.
- Gene ID
- 16160
- UniProt
- P43432
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