Recombinant mRuby antibody

Catalog No. ABIN6953292

Product Details

Target: mRuby

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Entacmaea quadricolor

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC)

Purpose: Unlabelled anti-mRuby sdAb with ectopic Cysteine on N- and C-Term for maleimide conjugation.

Specificity: MRUBY3 and mRUBY derivatives

Characteristics: The anti-mRUBY single-domain antibody has the same specificity as the respective labeled variant, but can be purchased here as unconjugated version.

Purification: Produced in: E.coli

Clone: 1F8

Affinity: Extinction coefficient (1/M*cm): 20190

Application Details

Application Notes: Uncoupled sdAb for doing maleimide conjugations. You can chose the position of the ectopic cysteine (N-terminal, C-terminal or both ends).
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Protocol: Maleimide Labelling Protocol

• Remove TCEP and exchange buffer to 10 mM KPi, 300 mM NaCl, 2 mM EDTA, pH 6.0 by size exclusion chromatography
• Before labelling, add 1/10 vol 1M Tris/HCl pH 8.0
• On ice, add 1.5- to 5- fold molar excess of fluorophore as maleimide
• Quickly vortex
• Overlay with Argon
• Place on ice for 90 min
• Separate excess of dye from the labelled sdAb e.g. by size exclusion chromatography

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water. Use unconjugated sdAbs immediately after reconstitution.

Buffer: lyophilized from 10 mM KPi, 300 mM NaCl, 2 mM EDTA, pH 6.0

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details

Target: mRuby