Recombinant Blue Fluorescent Protein antibody (AZDye 568)

Catalog No. ABIN6953241

Product Details anti-Blue Fluorescent Protein Antibody (AZDye 568)

Target: Blue Fluorescent Protein (BFP)

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Entacmaea quadricolor

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: This Blue Fluorescent Protein antibody is conjugated to AZDye 568

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Camelid sdAb anti-TagBFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 1H7

Specificity: Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor

Cross-Reactivity (Details): Does not cross-react with common GFP- or dsRed derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Clone: 1H7

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Application Details

Application Notes: Recommended dilution 1:500

Comment:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA. Please reconstitute with 200 µL of 50 % glycerol in deionized water. It is recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 5 µM.

Concentration: 5 μM

Buffer: 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. It is recommend storing the reconstituted protein at 4 °C.

Expiry Date: 12 months

Target Details for Blue Fluorescent Protein

Target: Blue Fluorescent Protein (BFP)

Alternative Name: TagBFP (BFP Products)