Recombinant SARS-CoV-2 Nucleocapsid antibody (AA 1-419)

Catalog No. ABIN6953156

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type: Recombinant Antibody

Fragment: scFv fragment

Binding Specificity: AA 1-419

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Human

Clonality: Chimeric

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA, Colloidal Gold Immunochromatography Assay (GICA)

Characteristics: Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293), It is a chimeric monoclonal antibody combining the mouse variable regions with the constant domains of human IgG1.
Monoclonal mouse (varialbe region)/human (kappa/IgG1 constant)chimeric antibody

Purification: Affinity-chromatography

Immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)

Clone: 1A6

Isotype: IgG1

Application Details

Application Notes: ELISA 1:10000-1:50000
GICA 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 % Glycerol, 0.01M PBS, pH 7.4, 0.03 % Proclin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Products)

Synonyms: nucleocapsid phosphoprotein antibody, N antibody

Target Type: Viral Protein

Background: Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3' end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine-arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.

UniProt: P0DTC9