Histone 3 antibody (H3K9me3)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K9me3
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA)
- Specificity
- Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 9 (H3K9me3), using a KLH-conjugated synthetic peptide.
- Cross-Reactivity
- Mouse (Murine), Yeast (Saccharomyces cerevisiae)
- Cross-Reactivity (Details)
- broad range
- Purification
- Peptide affinity purified
- Grade
- ChIP-seq Grade
- Immunogen
- synthetic peptide
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
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ChIP/ChIP-seq 1 μg:ChIP
ELISA 1:1,000
Dot Blot 1:20,000
Peptide array 1:5,000
WB 1:1,000
IF 1:250 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.9 μg/μL
- Buffer
- PBS, 0.05 % azide, 0.05 % ProClin300
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone 3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Background
- Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K9 is associated with inactive genomic regions, satellite repeats and ZNF gene repeats.
- UniProt
- P68431
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