Histone 3 antibody (H3K4me3)

Catalog No. ABIN6952351

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)

Specificity: Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide.

Cross-Reactivity: Arabidopsis, Daphnia, Maize/Corn (Zea mays), Mouse (Murine), Poplar (Populus), Rice (Oryza sativa), Tomato (Solanum lycopersicum), Trout, Zebrafish (Danio rerio)

Cross-Reactivity (Details): wide range

Purification: Peptide affinity purified

Grade: ChIP-seq Grade

Immunogen: synthetic peptide

Application Details

Application Notes: ChIP/ChIP-seq<1 μg:ChIP
ELISA 1:1,000
Dot Blot 1:10,000
Peptde Array 1:10,000
WB 1:1,000
IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.3 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Histone 3 antibody (ABIN6952351)

Schreiber, Mercier, Jiménez, Ye, García-Sánchez, Klein, Meunier, Ghimire, Birck, Jost, de Lichtenberg, Honoré, Serup, Gradwohl: "Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network." in: Molecular metabolism, Vol. 53, pp. 101313, (2021) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone 3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K4 is associated with activation of gene transcription.

UniProt: P68431