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Histone 3 antibody (H3K27me3)

Name: Histone 3 antibody (H3K27me3)
SKU: ABIN6952339
Price: 675.94 USD
Availability: InStock

ChIP-seq Grade H3 Reactivity: Human WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, SNAP-ChIP Host: Rabbit Polyclonal unconjugated

(1 reference)

Catalog No. ABIN6952339

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)
Binding Specificity
57

49

47

41

40

40

36

36

35

34

34

33

32

30

28

27

26

23

23

23

22

22

20

18

18

17

16

16

16

15

15

15

15

14

13

13

13

12

12

12

11

11

10

10

10

10

9

9

8

8
H3K27me3
Reactivity
1385

957

877

59

42

41

41

40

39

35

35

30

26

22

20

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human
Host
1203

239

8

5
Rabbit
Compatible Secondaries
Clonality
1127

327

1
Polyclonal
Conjugate
796

80

48

48

48

48

48

48

34

33

21

18

18

18

17

17

17

17

17

17

17

17

12

1
This Histone 3 antibody is un-conjugated
Application
1034

406

374

315

241

188

173

166

163

153

139

127

117

48

38

34

32

22

15

14

7

7

5

4

3

1

1

1
Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)

Specificity

Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Cross-Reactivity

Arabidopsis, Caenorhabditis elegans (C. elegans), Daphnia, Fruit Fly (Drosophila melanogaster), Maize/Corn (Zea mays), Mouse (Murine), Poplar (Populus), Red Algae (Chondrus), Tomato (Solanum lycopersicum)

Cross-Reactivity (Details)

wide range

Purification

Peptide affinity purified

Grade

ChIP-seq Grade

Immunogen

synthetic peptide

Top Product

Discover our top product H3 Primary Antibody
anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

ChIP/ChIP-seq 1 - 2 μg:ChIP
ELISA 1:100 - 1:500
Dot Blot 1:5,000
WB 1:1,000
IF 1:200

Restrictions

For Research Use only
Format

Liquid

Concentration

1.1 μg/μL

Buffer

PBS, 0.05 % azide, 0.05 % ProClin300

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C
Wang, Zhao, Liu, Wang, Qiao, Yi, Jiang, Kou, Zhao, Yin, Li, Wang, Jiang, Gao, Chen: "BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding." in: Protein & cell, (2022) (PubMed).
Target

Histone 3 (H3)

Alternative Name

Histone 3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.

UniProt

P68431