Taq DNA Polymerase antibody
Reactivity: Thermus aquaticus
PCR
Host: Mouse
Monoclonal
unconjugated
2 images
-
- Target See all Taq DNA Polymerase products
- Taq DNA Polymerase
- Reactivity
- Thermus aquaticus
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- The anti-Taq antibody inhibits the Taq DNA Polymerase at room temperature to increase specificity and sensitivity of the PCR.
- Specificity
-
- Inhibits polymerase activity until desired
- Enhances polymerase performance & stability under harsh cycling conditions
- No DNA contaminations detected with PCR
- Characteristics
- HotStart Antibody is a highly pure antibody that binds to Taq DNA Polymerase and thereby blocks its polymerase activity. At the very beginning of the thermal cycling protocol, the antibody is inactivated and with this ""Hot Start"", Taq DNA Polymerase regains its function. This highly controlled reaction start prevents non-specific amplification and grants maximum control over the reaction conditions.
- Purification
- Affinity Column
- Immunogen
- Taq DNA Polymerase
- Isotype
- IgG
-
- Application Notes
- Use 20 ng antibody for 1 Unit Taq DNA Polymerase
- Comment
-
For convenience, 5 U/μl Taq polymerase can be mixed with an equal volume of HotStart antibody, aliquoted and stored at -20°C until further use.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 5 U/μL
- Buffer
- TE-Buffer, pH 7.4
- Storage
- -20 °C
-
- Target
- Taq DNA Polymerase
- Alternative Name
- Taq Polymerase (Taq DNA Polymerase Products)
- Molecular Weight
- 94 kDa
-
You are here:
