tau antibody (Biotin)

Catalog No. ABIN6952089

Product Details anti-tau Antibody (Biotin)

Target: tau (tau Protein)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This tau antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Specificity: Expressed in neurons.,Detects Multiple Bands. Antibody detects monomer under denaturing conditions but preferentially detects fibril under native conditions (dot blot).

Cross-Reactivity: Human, Mouse

Purification: Protein G Purified

Immunogen: Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils

Clone: 1D5

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN6952089 was sufficient for detection of Tau 2N4R P301S Fibril in 20 ug of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau (tau Products)

Synonyms: DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, tau antibody, PHF-tau antibody, microtubule associated protein tau antibody, MAPT antibody

Background: Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).

NCBI Accession: NP_005901

UniProt: P10636