Topoisomerase I antibody
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- Target See all Topoisomerase I (TOP1) Antibodies
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Topoisomerase I antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))
- Cross-Reactivity
- Human, Mouse
- Purification
- Purified by Protein A.
- Immunogen
- Human Topoisomerase I between 1-100 amino acids
- Clone
- 1D1
- Isotype
- IgG
- Top Product
- Discover our top product TOP1 Primary Antibody
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anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibody
TOP1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1A1 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human WB, ELISA, IHC, IP, FACS Host: Rabbit Monoclonal 6D8 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (Internal Region) antibodyTOP1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (N-Term) antibodyTOP1 Reactivity: Human WB, FACS Host: Mouse Monoclonal 1291CT875-142-166 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 1-100) antibodyTOP1 Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 2-31), (N-Term) antibodyTOP1 Reactivity: Human WB, IF Host: Rabbit Polyclonal RB34034 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 358-501) antibodyTOP1 Reactivity: Human ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human, Rat WB, FACS Host: Mouse Monoclonal 1C6 unconjugated
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- Application Notes
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC() - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
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- Target
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- Alternative Name
- Topoisomerase I (TOP1 Products)
- Target Type
- Viral Protein
- Background
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Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1
Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
- Gene ID
- 7150
- UniProt
- P11387
- Pathways
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
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