SRP54 antibody

Catalog No. ABIN6945061

Product Details anti-SRP54 Antibody

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SRP54 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human SRP54 aa 1-200.

Clone: 1A4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SRP54

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Alternative Name: SRP54 (SRP54 Products)

Background:

Synonyms: Signal recognition particle 54 kDa protein, SRP54

Background: SRP54, a component of the signal recognition particle (SRP), recognizes the signal peptide of secretory proteins and interacts with the signal recognition particle receptor to target the ribosome and the associated nascent chain to the endoplasmic reticulum. By functional analysis showed that SRP54 binds to SRP RNA via the M-domain, but only in the presence of RNA-bound SRP19, and that it associates with the signal peptide of nascent polypeptide chains. RNA interaction requires the presence of a loop in the C-terminal M-domain. Signal peptide recognition most likely involves methionine-rich loops. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts the signal recognition particle receptor, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.

Gene ID: 6729

UniProt: P61011

Pathways: SARS-CoV-2 Protein Interactome