CHAF1B antibody
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- Target See all CHAF1B Antibodies
- CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This CHAF1B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))
- Cross-Reactivity
- Human
- Purification
- Purified by Protein A.
- Immunogen
- Recombinant protein corresponding to the C-terminus of Human p60 CAF1.
- Clone
- 9A7
- Isotype
- IgG
- Top Product
- Discover our top product CHAF1B Primary Antibody
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- Application Notes
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg) - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
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- Target
- CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))
- Alternative Name
- CHAF1B (CHAF1B Products)
- Synonyms
- CHAF1B antibody, wu:fd07c09 antibody, wu:fe36g06 antibody, wu:fv38g09 antibody, zgc:56096 antibody, CAF-1 antibody, CAF-IP60 antibody, CAF1 antibody, CAF1A antibody, CAF1P60 antibody, MPHOSPH7 antibody, MPP7 antibody, 2600017H24Rik antibody, C76145 antibody, CAF-I p60 antibody, CAF-Ip60 antibody, caf-1 antibody, CAF-1P60 antibody, chromatin assembly factor 1 subunit B antibody, chromatin assembly factor 1, subunit B antibody, chromatin assembly factor 1, subunit B (p60) antibody, chromatin assembly factor 1 subunit B L homeolog antibody, CHAF1B antibody, chaf1b antibody, Chaf1b antibody, chaf1b.L antibody
- Background
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Synonyms: Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, p60 CAF1
Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin-associated proteins 1 (HP1_, HP1_ and HP1_). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
- Gene ID
- 8208
- UniProt
- Q13112
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