CDC34 antibody

Catalog No. ABIN6942464

Product Details anti-CDC34 Antibody

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CDC34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Full length protein

Clone: 3C2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CDC34

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Alternative Name: Cdc34 (CDC34 Products)

Synonyms: E2-CDC34 antibody, UBC3 antibody, UBCH3 antibody, UBE2R1 antibody, AI327276 antibody, e2-cdc34 antibody, ubc3 antibody, ube2r1 antibody, CDC34 antibody, DNA6 antibody, Ube2r2 antibody, zgc:55452 antibody, fb34h09 antibody, wu:fb34h09 antibody, wu:fe17e09 antibody, zgc:91847 antibody, cell division cycle 34 antibody, cell division cycle 34 L homeolog antibody, SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 antibody, SCF E2 ubiquitin-protein ligase catalytic subunit antibody, cell division cycle 34 homolog (S. cerevisiae) a antibody, cell division cycle 34 homolog (S. cerevisiae) b antibody, CDC34 antibody, Cdc34 antibody, cdc34.L antibody, cdc34 antibody, cdc34a antibody, cdc34b antibody

Background:

Synonyms: Ubiquitin-conjugating enzyme E2 R1, (E3-independent) E2 ubiquitin-conjugating enzyme R1, E2 ubiquitin-conjugating enzyme R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1, CDC34

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421).

Gene ID: 997

UniProt: P49427