Recombinant BrdU antibody

Catalog No. ABIN6941451

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Antibody Type: Recombinant Antibody

Reactivity: Please inquire

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Neutralization (Neut), Staining Methods (StM)

Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purification: Purified by Protein A/G

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Clone: BRD2888R

Isotype: IgG

Application Details

Application Notes:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC (Cat. #AP-9008)]Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: Bromodeoxyuridine (BrdU) (Proliferation Marker) (BrdU Products)

Target Type: Chemical

Molecular Weight: 307Da