Keratin 77 antibody
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- Target See all Keratin 77 (KRT77) Antibodies
- Keratin 77 (KRT77)
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Reactivity
- Human, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Keratin 77 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- Cross-Reactivity (Details)
- Shows broad species reactivity.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human KRT77 protein
- Clone
- KRTL-1077
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product KRT77 Primary Antibody
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anti-Keratin 77 (KRT77) antibody
KRT77 Reactivity: Human, Rat, Mouse, Rabbit, Cow, Dog, Chicken, Monkey, Turtle IHC, WB, IF, FACS, StM Host: Mouse Monoclonal AE-1 unconjugated
anti-Keratin 77 (KRT77) (Internal Region) antibodyKRT77 Reactivity: Human, Rat IHC, ELISA, WB Host: Rabbit Polyclonal unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 Reactivity: Human IHC, StM Host: Rabbit Monoclonal KRTL-1577R unconjugated Recombinant Antibody
anti-Keratin 77 (KRT77) (AA 1-578) antibodyKRT77 Reactivity: Human IHC, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Positive Control: Skin, Squamous cell carcinoma (SCC).
Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Keratin 77 (KRT77)
- Alternative Name
- KRT77 (KRT77 Products)
- Molecular Weight
- 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
- Gene ID
- 374454
- UniProt
- Q7Z794
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