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Keratin 77 antibody

KRT77 Reactivity: Human, Rat, Cow, Dog, Mouse, Rabbit, Chicken, Monkey, Turtle IHC, WB, IF, FACS, StM Host: Mouse Monoclonal AE-1 unconjugated
Catalog No. ABIN6941429
  • Target See all Keratin 77 (KRT77) Antibodies
    Keratin 77 (KRT77)
    Reactivity
    • 25
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Cow, Dog, Mouse, Rabbit, Chicken, Monkey, Turtle
    Host
    • 18
    • 7
    Mouse
    Clonality
    • 16
    • 9
    Monoclonal
    Conjugate
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 77 antibody is un-conjugated
    Application
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    Specificity
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
    Purification
    Purified by Protein A/G
    Immunogen
    Human epidermal keratin
    Clone
    AE-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product KRT77 Primary Antibody
  • Application Notes

    Positive Control: Skin or squamous cell carcinoma (IHC).

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Keratin 77 (KRT77)
    Alternative Name
    KRT77 (KRT77 Products)
    Synonyms
    K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody
    Molecular Weight
    40-56.5kDa
    Gene ID
    374454
    UniProt
    Q7Z794
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