Macrophage / Histiocytoma Marker antibody
Reactivity: Human
IHC, StM
Host: Mouse
Monoclonal
D11
unconjugated
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- Target See all Macrophage / Histiocytoma Marker products
- Macrophage / Histiocytoma Marker
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Macrophage / Histiocytoma Marker antibody is un-conjugated
- Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- In Western blotting, it detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
- No Cross-Reactivity
- Mouse (Murine), Pig (Porcine), Rat (Rattus)
- Purification
- Purified by Protein A/G
- Immunogen
- Membrane preparation from human hepatocytes
- Clone
- D11
- Isotype
- IgG1 kappa
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anti-Macrophage / Histiocytoma Marker antibody
Reactivity: Human IHC, StM Host: Mouse Monoclonal LN-5 unconjugated
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- Application Notes
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Positive Control: Liver or Histiocytoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Macrophage / Histiocytoma Marker
- Alternative Name
- Macrophage & Histiocytoma Marker (Macrophage / Histiocytoma Marker Products)
- Molecular Weight
- 125kDa -135kDa
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