Recombinant MHC Class II HLA-DP/DQ/DR antibody
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- Target See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Coating (Coat), ELISA, Staining Methods (StM)
- Specificity
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
- Purification
- Purified by Protein A/G
- Immunogen
- Non-T, non-B human acute lymphoblastic leukemia REH6 cells
- Clone
- RHLA-Pan-3475
- Isotype
- IgG1 kappa
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- Application Notes
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Positive Control: Raji cells. Tonsil or lymph node.
Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- Alternative Name
- HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Products)
- Molecular Weight
- 33-35kDa
- Gene ID
- 3115, 3117, 3122
- UniProt
- P04440, P01908, P01909, P01920, P01903
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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