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Recombinant MHC Class II HLA-DP/DQ/DR antibody

HLA-DP/DQ/DR Reactivity: Human FACS, IF, IHC, Coat, ELISA, StM Host: Mouse Monoclonal rHLA-Pan-3475 unconjugated Recombinant Antibody
Catalog No. ABIN6941388
  • Target See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
    MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 18
    • 1
    Human
    Host
    • 16
    • 2
    Mouse
    Clonality
    • 18
    Monoclonal
    Conjugate
    • 10
    • 4
    • 2
    • 2
    This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
    Application
    • 16
    • 7
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Coating (Coat), ELISA, Staining Methods (StM)
    Specificity
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
    Purification
    Purified by Protein A/G
    Immunogen
    Non-T, non-B human acute lymphoblastic leukemia REH6 cells
    Clone
    RHLA-Pan-3475
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: Raji cells. Tonsil or lymph node.

    Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
    Alternative Name
    HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Products)
    Molecular Weight
    33-35kDa
    Gene ID
    3115, 3117, 3122
    UniProt
    P04440, P01908, P01909, P01920, P01903
    Pathways
    Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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