pan Keratin antibody
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- Target See all pan Keratin (panKRT) Antibodies
- pan Keratin (panKRT)
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Reactivity
- Human, Rat, Mouse, Cow, Pig, Frog, Guinea Pig, Goat, Marmoset
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This pan Keratin antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- Purification
- Purified by Protein A/G
- Immunogen
- Keratin-enriched preparation from cultured human A431
- Clone
- SPM583
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product panKRT Primary Antibody
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- Application Notes
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Positive Control: A431 cells, Skin, Colon carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-4 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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