KRT antibody

Catalog No. ABIN6941353

Product Details anti-KRT Antibody

Target: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Cow, Monkey, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Purification: Purified by Protein A/G

Immunogen: Human epidermal keratins

Clone: Cocktail PAN-CK

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT

Target: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Alternative Name: KRT (KRT126P Products)

Synonyms: KRT antibody, keratin 126 pseudogene antibody, KRT126P antibody

Molecular Weight: 40-67kDa (Multiple)

Gene ID: 374454, 51350

UniProt: Q7Z794, Q01546