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KRT antibody

KRT126P Reactivity: Human, Mouse, Rat, Dog, Chicken, Cow, Monkey, Rabbit FACS, IHC, StM Host: Mouse Monoclonal Cocktail PAN-CK unconjugated
Catalog No. ABIN6941353
  • Target See all KRT (KRT126P) products
    KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
    Reactivity
    Human, Mouse, Rat, Dog, Chicken, Cow, Monkey, Rabbit
    Host
    • 4
    • 3
    Mouse
    Clonality
    • 5
    • 2
    Monoclonal
    Conjugate
    • 7
    This KRT antibody is un-conjugated
    Application
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
    Purification
    Purified by Protein A/G
    Immunogen
    Human epidermal keratins
    Clone
    Cocktail PAN-CK
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
    Alternative Name
    KRT (KRT126P Products)
    Synonyms
    KRT antibody, keratin 126 pseudogene antibody, KRT126P antibody
    Molecular Weight
    40-67kDa (Multiple)
    Gene ID
    374454, 51350
    UniProt
    Q7Z794, Q01546
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