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Biotin antibody

WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated
Catalog No. ABIN6941338
  • Target See all Biotin products
    Biotin
    Reactivity
    Please inquire
    Host
    • 56
    • 32
    • 28
    Mouse
    Clonality
    • 75
    • 34
    Monoclonal
    Conjugate
    • 28
    • 11
    • 8
    • 7
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Biotin antibody is un-conjugated
    Application
    • 54
    • 48
    • 43
    • 35
    • 32
    • 19
    • 14
    • 12
    • 12
    • 12
    • 9
    • 9
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)
    Specificity
    It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
    Purification
    Purified by Protein A/G
    Immunogen
    Biotinylated sheep immunoglobulin
    Clone
    Hyb-8
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: Biotinylated proteins in solution or on tissues.

    Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Biotin
    Alternative Name
    Biotin (Vitamin B7 or Vitamin H) (Biotin Products)
    Target Type
    Chemical
    Molecular Weight
    244Da
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