Testosterone antibody
ELISA, IHC, Coat, RIA, StM
Host: Mouse
Monoclonal
4E1G2
unconjugated
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- Target See all Testosterone Antibodies
- Testosterone
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Testosterone antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Coating (Coat), Radioimmunoassay (RIA), Staining Methods (StM)
- Specificity
- This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100 % , 11-beta-hydroxy testosterone 3.3 %, 17-alpha-methyl testosterone
- Purification
- Purified by Protein A/G
- Immunogen
- Testosterone 3 CMO conjugated to BSA
- Clone
- 4E1G2
- Isotype
- IgG1 kappa
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- Application Notes
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Positive Control: Interstitial or Leydid cells in testis.
Known Application: ELISA (For coating, order Ab without BSA), Radioimmnoassay (RIA),Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Testosterone
- Abstract
- Testosterone Products
- Target Type
- Hormone
- Molecular Weight
- 288.42Da
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