CD68 antibody (AA 150-301)

Catalog No. ABIN6941245

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Binding Specificity: AA 150-301

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunostaining (ISt), Staining Methods (StM), Coating (Coat)

Specificity: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of humanCD68 protein (around aa 150-301) (exact sequence is proprietary)

Clone: LAMP4-1830

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: U87MG, Tonsil, Lymph Node or Spleen.

Known Application: Flow Cytometry (1-2 μg/million cells),ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Synonyms: Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody

Molecular Weight: 110kDa

Gene ID: 968

UniProt: P34810