Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD40 Ligand antibody (AA 108-261)

CD40LG Reactivity: Human FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal CD40LG-2763 unconjugated
Catalog No. ABIN6941189
  • Target See all CD40 Ligand (CD40LG) Antibodies
    CD40 Ligand (CD40LG)
    Binding Specificity
    • 16
    • 10
    • 8
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 108-261
    Reactivity
    • 152
    • 81
    • 34
    • 9
    • 8
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 97
    • 65
    • 22
    • 15
    • 12
    • 1
    Mouse
    Clonality
    • 112
    • 94
    • 4
    Monoclonal
    Conjugate
    • 105
    • 23
    • 21
    • 14
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD40 Ligand antibody is un-conjugated
    Application
    • 109
    • 101
    • 52
    • 50
    • 39
    • 29
    • 20
    • 17
    • 14
    • 14
    • 13
    • 13
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary)
    Clone
    CD40LG-2763
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD40LG Primary Antibody
  • Application Notes

    Positive Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC).

    Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    CD40 Ligand (CD40LG)
    Alternative Name
    CD40LG (CD40LG Products)
    Synonyms
    CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody
    Background
    CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.
    Molecular Weight
    36kDa
    Gene ID
    959
    UniProt
    P29965
    Pathways
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
You are here:
Support