CD40 antibody (AA 29-107)

Catalog No. ABIN6941187

Product Details anti-CD40 Antibody

Target: CD40

Binding Specificity: AA 29-107

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Staining Methods (StM), Coating (Coat)

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)

Clone: C40-2383

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: U2OS, L-428 cells. Tonsil, Spleen or Thymus.

Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD40

Target: CD40

Alternative Name: CD40 (CD40 Products)

Synonyms: Bp50 antibody, CDW40 antibody, TNFRSF5 antibody, p50 antibody, AI326936 antibody, GP39 antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TRAP antibody, Tnfrsf5 antibody, TNFSF5 antibody, CD40 molecule antibody, CD40 antigen antibody, CD40 antibody, Cd40 antibody

Background: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Molecular Weight: 43kDa

Gene ID: 958

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints