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CD19 antibody (AA 96-281)

CD19 Reactivity: Human FACS, IHC, ELISA, Coat, StM Host: Mouse Monoclonal CD19-3116 unconjugated
Catalog No. ABIN6941101
  • Target See all CD19 Antibodies
    CD19 (CD19 Molecule (CD19))
    Binding Specificity
    • 23
    • 16
    • 16
    • 14
    • 10
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 96-281
    Reactivity
    • 280
    • 147
    • 41
    • 29
    • 19
    • 10
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 169
    • 130
    • 41
    • 4
    Mouse
    Clonality
    • 225
    • 115
    • 2
    Monoclonal
    Conjugate
    • 120
    • 22
    • 21
    • 20
    • 17
    • 8
    • 8
    • 7
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD19 antibody is un-conjugated
    Application
    • 251
    • 113
    • 69
    • 65
    • 50
    • 47
    • 23
    • 21
    • 14
    • 14
    • 9
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant fragment of human CD19 protein (around aa96-281) (exact sequence is proprietary)
    Clone
    CD19-3116
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD19 Primary Antibody
  • Application Notes

    Positive Control: Raji cells (FACS). Tonsil, Lymph Node or Spleen (IHC).

    Known Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    CD19 (CD19 Molecule (CD19))
    Alternative Name
    CD19 (CD19 Products)
    Background
    CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.
    Molecular Weight
    95kDa
    Gene ID
    930
    UniProt
    P15391
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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