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CD14 antibody (AA 25-148)

Name: CD14 antibody (AA 25-148)
Brand: antibodies-online
SKU: ABIN6941093
Price: 597.38 USD
Availability: InStock

CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

Flow Cytometric Analysis of A549 cells using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 antibody (AA 25-148))

FACS

Western Blot Analysis of A549 cell lysate using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 antibody (AA 25-148))

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD14 antibody (AA 25-148))

PAr

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 antibody (AA 25-148))

IHC

Formalin-fixed, paraffin-embedded human Tonsil stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 antibody (AA 25-148))

IHC

SDS-PAGE Analysis Purified CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 antibody (AA 25-148))

SDS

6 images

Catalog No. ABIN6941093

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
8

6

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 25-148
Reactivity
221

37

30

25

22

18

16

15

14

13

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Human
Host
191

52

16

3

1
Mouse
Compatible Secondaries
Clonality
209

54
Monoclonal
Conjugate
107

30

22

17

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
196

77

56

49

45

44

20

13

13

11

6

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Specificity

Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Purification

Purified by Protein A/G

Immunogen

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Clone

LPSR-2385

Isotype

IgG1 kappa

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CD14 Reactivity: Human IHC, ELISA, ICC Host: Mouse Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 Biotin

Application Notes

Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date

24 months
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Molecular Weight

55kDa

Gene ID

929

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades