Aurora Kinase B antibody (AA 89-251)

Catalog No. ABIN6941045

Product Details anti-Aurora Kinase B Antibody

Target: Aurora Kinase B (AURKB)

Binding Specificity: AA 89-251

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: Recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)

Clone: AURKB-1592

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB (AURKB Products)

Synonyms: AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, PPP1R48 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, AIRK2 antibody, AL022959 antibody, Aik2 antibody, Aim1 antibody, Ark2 antibody, STK-1 antibody, Stk12 antibody, Stk5 antibody, Aur B antibody, Aurora B antibody, Aurora-B antibody, CG6620 antibody, DmAIRK2 antibody, DmAurB antibody, DmAuroraB antibody, Dmel\\CG6620 antibody, IAL antibody, IAL1 antibody, Ial antibody, aurB antibody, aurora B antibody, ARK-2 antibody, XAIRK2 antibody, aik2 antibody, aim-1 antibody, aim1 antibody, airk2 antibody, ark2 antibody, ipl1 antibody, stk12 antibody, stk5 antibody, CHUNP6896 antibody, fa09g06 antibody, stka antibody, wu:fa09g06 antibody, aurkb antibody, AIRK2-B antibody, XAIRK2-B antibody, aurkb-a antibody, aurkb-b antibody, xAurora-B-B antibody, aurora kinase B antibody, aurora B antibody, aurora kinase B S homeolog antibody, AURKB antibody, Aurkb antibody, aurB antibody, aurkb antibody, aurkb.S antibody

Molecular Weight: 39kDa

Gene ID: 9212

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades