CD3 epsilon antibody
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- Target See all CD3 epsilon (CD3E) Antibodies
- CD3 epsilon (CD3E)
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Reactivity
- Human, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD3 epsilon antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act), Proliferation Assay (ProA)
- Specificity
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
- Immunogen
- Stimulated human leukocytes
- Clone
- CRIS-7
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD3E Primary Antibody
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anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E Reactivity: Human, Mouse, Rat WB, IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E Reactivity: Human, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig, Chicken FACS, WB, IF Host: Mouse Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reactivity: Human, Mouse, Rat FACS, IF, Func Host: Mouse Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E Reactivity: Human FACS, WB, IHC, ELISA, Coat, StM Host: Mouse Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E Reactivity: Human WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Positive Control: Jurkat cells. Tonsil and lymph node.
Known Application: Induce T cell activation and proliferation (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Molecular Weight
- 20kDa
- Gene ID
- 916
- UniProt
- P07766
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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