CD3 epsilon antibody (AA 23-119)

Catalog No. ABIN6941022

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Binding Specificity: AA 23-119

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Purification: Purified by Protein A/G

Immunogen: A recombinant human CD3e protein fragment (around aa 23-119) (exact sequence is proprietary)

Clone: C3e-1931

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat cells. Tonsil or lymph node.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E (CD3E Products)

Molecular Weight: 20kDa

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway