Cyclin B2 antibody

Catalog No. ABIN6941010

Product Details anti-Cyclin B2 Antibody

Target: Cyclin B2 (CCNB2)

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Clone: X29-2

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cyclin B2

Target: Cyclin B2 (CCNB2)

Alternative Name: CCNB2 (CCNB2 Products)

Synonyms: HsT17299 antibody, CycB2 antibody, sb:cb303 antibody, cycB2 antibody, LOC100136691 antibody, C-B2 antibody, cyclinb2 antibody, hst17299 antibody, LOC100282653 antibody, cyclin B2 antibody, cyclin B2 S homeolog antibody, CCNB2 antibody, Ccnb2 antibody, ccnb2 antibody, LOC100136691 antibody, LOC100282653 antibody, ccnb2.S antibody

Background: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Molecular Weight: 51kDa

Gene ID: 9133

UniProt: O95067

Pathways: Cell Division Cycle, M Phase