Caldesmon antibody

Catalog No. ABIN6940938

Product Details anti-Caldesmon Antibody

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Specificity: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Purification: Purified by Protein A/G

Immunogen: Crude human uterus extract

Clone: H-CALD

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: CALD1 (CALD1 Products)

Synonyms: CDM antibody, H-CAD antibody, HCAD antibody, L-CAD antibody, LCAD antibody, NAG22 antibody, l-Cad antibody, 4833423D12Rik antibody, AI195384 antibody, AV071549 antibody, AW536160 antibody, C920027I18Rik antibody, CALD1 antibody, Caldesmon antibody, caldesmon 1 antibody, CALD1 antibody, Cald1 antibody

Molecular Weight: 150kDa

Gene ID: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction