CA9 antibody
-
- Target See all CA9 Antibodies
- CA9 (Carbonic Anhydrase IX (CA9))
-
Reactivity
- Human, Horse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CA9 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human CA9 protein
- Clone
- CA9-781
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CA9 Primary Antibody
-
-
- Application Notes
-
Positive Control: Normal kidney or renal cell carcinoma.
Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- CA9 (Carbonic Anhydrase IX (CA9))
- Alternative Name
- CA9 (CA9 Products)
- Synonyms
- CA9 antibody, CAIX antibody, Ca9 antibody, MN/CA9 antibody, MN antibody, carbonic anhydrase 9 antibody, CA9 antibody, CAH9 antibody, Car9 antibody
- Molecular Weight
- 55kDa
- Gene ID
- 768
- UniProt
- Q16790
-