VWF antibody (AA 845-949)
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- Target See all VWF Antibodies
- VWF (Von Willebrand Factor (VWF))
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Binding Specificity
- AA 845-949
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This VWF antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)
- Clone
- 3E2D10
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VWF Primary Antibody
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- Application Notes
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Positive Control: HUVEC cells. Tonsil.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- VWF (Von Willebrand Factor (VWF))
- Alternative Name
- VWF (VWF Products)
- Synonyms
- VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody
- Background
- Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
- Molecular Weight
- 250kDa
- Gene ID
- 7450
- UniProt
- P04275
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