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Recombinant Vimentin antibody

VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody
Catalog No. ABIN6940885
  • Target See all Vimentin (VIM) Antibodies
    Vimentin (VIM)
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 341
    • 133
    • 130
    • 60
    • 60
    • 49
    • 42
    • 37
    • 29
    • 21
    • 20
    • 13
    • 13
    • 8
    • 8
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 194
    • 130
    • 11
    • 6
    • 6
    • 5
    • 2
    Rabbit
    Clonality
    • 211
    • 142
    Monoclonal
    Conjugate
    • 203
    • 19
    • 14
    • 14
    • 10
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Vimentin antibody is un-conjugated
    Application
    • 285
    • 130
    • 105
    • 100
    • 96
    • 64
    • 46
    • 40
    • 39
    • 33
    • 23
    • 19
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant full-length human Vimentin protein
    Clone
    VIM-1937R
    Isotype
    IgG
    Top Product
    Discover our top product VIM Primary Antibody
  • Application Notes

    Positive Control: U-87, A375, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

    Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Vimentin (VIM)
    Alternative Name
    VIM (VIM Products)
    Synonyms
    CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
    Molecular Weight
    57-60kDa
    Gene ID
    7431
    UniProt
    P08670
    Pathways
    Caspase Cascade in Apoptosis
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