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TYR antibody

TYR Reactivity: Human IF, IHC, FACS, StM Host: Mouse Monoclonal T311 unconjugated
Catalog No. ABIN6940824
  • Target See all TYR Antibodies
    TYR (Tyrosinase (TYR))
    Reactivity
    • 95
    • 22
    • 20
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 61
    • 35
    Mouse
    Clonality
    • 52
    • 45
    Monoclonal
    Conjugate
    • 52
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TYR antibody is un-conjugated
    Application
    • 48
    • 37
    • 27
    • 27
    • 21
    • 20
    • 18
    • 13
    • 13
    • 9
    • 7
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
    Specificity
    Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant tyrosinase protein
    Clone
    T311
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product TYR Primary Antibody
  • Application Notes

    Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Melanoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    TYR (Tyrosinase (TYR))
    Alternative Name
    TYR (TYR Products)
    Synonyms
    sandy antibody, zgc:109705 antibody, TYR antibody, TYRO antibody, tyr antibody, LOC100136546 antibody, CMM8 antibody, OCA1A antibody, OCAIA antibody, SHEP3 antibody, albino antibody, c antibody, skc35 antibody, C antibody, tyrosinase antibody, TYRosinase antibody, tyrosinase S homeolog antibody, tyr antibody, tyr-5 antibody, tyr.S antibody, TYR antibody, Celal_3121 antibody, Halhy_4121 antibody, Mesop_2736 antibody, LOC100136546 antibody, Tyr antibody
    Molecular Weight
    70-80kDa
    Gene ID
    7299
    UniProt
    P14679
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