TYMS antibody (AA 60-174)

Catalog No. ABIN6940822

Product Details anti-TYMS Antibody

Target: TYMS (Thymidylate Synthetase (TYMS))

Binding Specificity: AA 60-174

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)

Specificity: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Clone: TYMS-1884

Isotype: IgG2c kappa

Application Details

Application Notes:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TYMS (TYMS Products)

Target Type: Viral Protein

Molecular Weight: 36kDa

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases