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TYMS antibody

TYMS Reactivity: Human IF, FACS, IHC, ISt, RIA, StM Host: Mouse Monoclonal TS106 unconjugated
Catalog No. ABIN6940818
  • Target See all TYMS Antibodies
    TYMS (Thymidylate Synthetase (TYMS))
    Reactivity
    • 92
    • 29
    • 14
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 50
    • 41
    • 1
    Mouse
    Clonality
    • 48
    • 44
    Monoclonal
    Conjugate
    • 46
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TYMS antibody is un-conjugated
    Application
    • 52
    • 41
    • 33
    • 23
    • 19
    • 18
    • 17
    • 17
    • 14
    • 13
    • 9
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)
    Specificity
    It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant full-length human TYMS protein.
    Clone
    TS106
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product TYMS Primary Antibody
  • Application Notes

    Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    TYMS (Thymidylate Synthetase (TYMS))
    Alternative Name
    TYMS (TYMS Products)
    Target Type
    Viral Protein
    Molecular Weight
    36kDa
    Gene ID
    7298
    UniProt
    P04818
    Pathways
    Mitotic G1-G1/S Phases
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