C4B antibody

Catalog No. ABIN6940809

Product Details anti-C4B Antibody

Target: C4B (C4b) (Complement Component C4b (C4b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C4B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Specificity: This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Purification: Purified by Protein A/G

Immunogen: Recombinant human Complement 4d protein

Clone: SPM545

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Rejected Renal Transplant Tissue.

Known Application: Immunofluorescence (1:50-1:100), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for C4B

Target: C4B (C4b) (Complement Component C4b (C4b))

Alternative Name: C4B (C4b Products)

Molecular Weight: 192kDa (predicted)

Gene ID: 720, 721

Pathways: Complement System