Recombinant Thrombomodulin antibody (AA 69-194)

Catalog No. ABIN6940729

Product Details anti-Thrombomodulin Antibody

Target: Thrombomodulin (THBD)

Antibody Type: Recombinant Antibody

Binding Specificity: AA 69-194

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunostaining (ISt), Staining Methods (StM)

Specificity: It recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)

Clone: RTHBD-1591

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: THP1 cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: THBD (THBD Products)

Synonyms: AHUS6 antibody, BDCA3 antibody, CD141 antibody, THPH12 antibody, THRM antibody, TM antibody, AI385582 antibody, THBD antibody, HAST antibody, HAST3 antibody, M-PST antibody, ST1A3/ST1A4 antibody, ST1A5 antibody, STM antibody, TL-PST antibody, thrombomodulin antibody, sulfotransferase family 1A member 3 antibody, THBD antibody, Thbd antibody, SULT1A3 antibody

Molecular Weight: ~100kDa

Gene ID: 7056

UniProt: P07204